鸡γ-干扰素的克隆和序列分析

Cloning and Sequence Analysis of Chicken Interferon-gamma

根据GenBank发表的鸡γ-干扰素核苷酸序列,使用primer 5设计一对特异性引物,通过RT-PCR技术从ConA诱导培养的鸡脾脏淋巴细胞中克隆出鸡γ-干扰素基因并对其进行测序,测序结果表明,鸡γ-干扰素基因全长495bp,具有一个完整的开放阅读框,编码164个氨基酸,与国外发表的序列比较,两序列间同源性为100%.计算机软件对鸡γ-干扰素编码的氨基酸序列进行了抗原性分析,结果表明鸡γ-干扰素具有良好的免疫原性.

Based on chicken interferon - gamma （ chicken IFN - γ） cDNA sequence published by GenBank, a pair of special primers were designed by computer software. A 495bp DNA fragment was amplified by RT =PCR using total RNA template extracted from chicken spleen lymphocyte that stimulate 24 hour by 1 milligram per milliliter con- centration CoA. In order to identify this gene, the nucleotide was sequenced and compared with existing data in the GenBank published by Weining, K.C. The result indicated this chicken IFN -γ gene included an open reading frame （OR.F） which encoded 164 amino acids residues, and had a 100% homology with chicken IFN -γ gene in C, enBank, DNAstar soft analyze antigenicity of the chicken IFN - γprotein. The result shows the chicken IFN - γprotein has beter immunogenic.