摘要
根据GenBank发表的鸡γ-干扰素核苷酸序列,使用primer 5设计一对特异性引物,通过RT-PCR技术从ConA诱导培养的鸡脾脏淋巴细胞中克隆出鸡γ-干扰素基因并对其进行测序,测序结果表明,鸡γ-干扰素基因全长495bp,具有一个完整的开放阅读框,编码164个氨基酸,与国外发表的序列比较,两序列间同源性为100%.计算机软件对鸡γ-干扰素编码的氨基酸序列进行了抗原性分析,结果表明鸡γ-干扰素具有良好的免疫原性.
Based on chicken interferon - gamma ( chicken IFN - γ) cDNA sequence published by GenBank, a pair of special primers were designed by computer software. A 495bp DNA fragment was amplified by RT =PCR using total RNA template extracted from chicken spleen lymphocyte that stimulate 24 hour by 1 milligram per milliliter con- centration CoA. In order to identify this gene, the nucleotide was sequenced and compared with existing data in the GenBank published by Weining, K.C. The result indicated this chicken IFN -γ gene included an open reading frame (OR.F) which encoded 164 amino acids residues, and had a 100% homology with chicken IFN -γ gene in C, enBank, DNAstar soft analyze antigenicity of the chicken IFN - γprotein. The result shows the chicken IFN - γprotein has beter immunogenic.
出处
《红河学院学报》
2007年第2期10-13,共4页
Journal of Honghe University
基金
红河学院校级课题项目(XS205026)
关键词
鸡γ-干扰素
克隆
表达
chicken interferon - gamma( chicken IFN - γ gene
cloning
sequence analysis