罗曼竹芋的组培快繁技术研究

Study on Techniques of Tissue Culture and Rapid Propagation of Calathea Leopardina

以罗曼竹芋茎段作为外植体,进行了各个阶段的培养基筛选试验,结果表明,MS+6.0 mg/L 6-BA+0.5 mg/L NAA培养基诱导茎节芽萌发的效果最好,芽萌发率为90.9%;继代培养基以MS+10.0 mg/L 6-BA最为适宜;生根培养基以1/2 MS+1.0 mg/L NAA效果最好,生根率为92.9%。

The lateral stem culture of Calathea Leopardina were used as explant, the experiments was carried and media for various culture stages were selected. The results showed that the optimal media were MS＋ 6, 0 mg/L 6, 0-BA ＋ 0.5 mg/L NAA for buds inducing, the boureon rate of buds was 90. 9% ; MS＋10. 0 mg/L 6-BA for propagation and 1/2 MS＋ 1.0 mg/L NAA for inducing roots formation respectively,the rooting rate was 92.9%.