人脑胶质瘤逃避免疫监视机制的研究

Mechanism of Evading Surveillance in Gliomas

为研究胶质瘤逃避免疫监视的机制，检测了胶质瘤体外细胞系培养上清（SN）抗PHA－P刺激正常人及胶质瘤患者外周血淋巴细胞增殖的作用。证实了胶质瘤可自分泌免疫抑制因子。经抗TGFβ2单抗中和SN试验，TGFβ2免疫组化染色及Northern杂交进一步表明该因子主要成分为TGFβ2并对胶质瘤患者血浆皮质醇，生长激素及儿茶酚胺含量及细胞免疫功能进行检测，发现血浆去甲肾上腺素显著降低，其水平与CD8亚群％呈负相关，与CD4／CD8比值及淋巴细胞增殖率呈正相关。初步提示存在胶质瘤的情况下，神经－免疫调控紊乱，可能起着抑制性调节作用。

The effect of supernatant seperated from cultured media of malignant glioma cell lines on the proliferation of peripheral blood lymphocytes (PBL)from healthy subjects and patients with glioma activated by mitogen PHA-P were studied. It was found that the glioma cells could secrete a T-cell supressor factor in an autocrine fashion which was identified further as the transforming growth factor B2. In addition,the plasma cortisol,growth hormone and catecholamine as well as the cellular immunity of patients with glioma were determined. It was demonstrated that the plasma level of norepinephrine in glioma patients was significantly declined and correlated well with the suppression of cellular immunity themselves. This finding has not been reported previously and suggests that the neuroimmuno-modulation may be disturbed in the presence of cerebral glioma.