应用聚合酶链反应检测自然流产组织中沙眼衣原体和解脲支原体

Detection of Chlamydia Trachomatis and Ureaplasm Urealyticum from Aborted Tissues by Polymerase Chain Reaction Technique

目的：研究沙眼衣原体（ＣＴ）和解脲支原体（ＵＵ）对流产的影响。方法：应用聚合酶链反应（ＰＣＲ）技术分别对４５例自然流产患者（流产组）和２５例人工流产患者（对照组）的胚胎组织进行ＣＴ和ＵＵ检测。结果：流产组ＣＴ和ＵＵ的检出率分别为４２．２％和３３．３％，与对照组比较，差异有极显著性（Ｐ＜０．０１，Ｐ＜０．０２５），ＣＴ和ＵＵ复合感染率为８．９％。结论：ＣＴ和ＵＵ是引起自然流产的主要感染原。ＰＣＲ技术可作为临床诊断ＣＴ和ＵＵ的依据。

Objective: To study the prevalence of chlamydia trachomatis (CT) and ureaplasm urealyticum (UU) infection in spontaneous abortion patients. Methods: CT and UU in aborted tissues were determined by polymerase chain reaction (PCR) technique in 45 spontaneous abortion patients (abortion group) and 25 artificial abortion cases (control group). Results: The detective rate of CT and UU was 42.2% and 33.3% respectively in the abortion group. As compared with the control group significant difference ( P<0.01, P<0.025) was shown. The detection rate of mixed CT and UU is 8.9%. Conclusion: CT and UU are important infection factors which may cause spontaneous abortion. PCR examination of the aborted tissue can submit an accurate, rapid microorganic diagnosis of CT and (or) UU infection.