梅毒螺旋体检测方法的临床评价

Clinical evaluation of methods for detection of Treponema pallidum infection

目的探讨三种血清梅毒螺旋体检测方法的应用价值。方法应用快速血浆反应素试验(RPR)、酶联免疫吸附试验(ELISA)、梅毒螺旋体明胶凝集试验(TPPA)分别检测602份标本,其中61例确诊为梅毒感染。荧光定量PCR(FQ-PCR)方法对RPR阳性标本进行检测。结果ELISA法与TPPA法检测结果之间无显著性差异(P﹥0.05),二者敏感性和特异性分别为95.1%、97.8%和91.8%、96.9%;RPR与ELISA法、TPPA法之间有显著性差异(P﹤0.05);FQ-PCR法检测结果均为阴性。结论ELISA法和TPPA法是较好的血清梅毒螺旋体的检测方法。FQ-PCR法检测的阳性率低。

Objective To investigate the application value of four methods for detecting Treponema pallidum infection. Methods Three methods were used to test the serum of 602 cases. Sixty one of these cases were diagnosed as syphilis. The three methods were enzyme linked immunosorbent assay （ELISA）, Treponema pallidum particle assay （TPPA） and rapid plasma regain test （RPR）. 134 positive samples detected by RPR were re-examined by Fluorescence quantitative PCR （FQ- PCR） method. Results There was no obvious difference between ELISA and TPPA test results（P 〉 0.05）. The sensibility detected by ELISA and TPPA were 95.1% and 97.8%, while the specificity was 91.8% and 96.9%. There was significant difference between RPR and ELISA or TPPA （P 〈 0.05）. But those cases detected by FQ-PCR were negative. Conclusions Both ELISA and TPPA methods are good methods for determination of Treponema pallidum infection. The positive rate of FQ-PCR is low in the detection of Treponema pallidum.