用离子交换法分离苹果汁中果酸的技术研究

Technology for separation of fruit acid from apple juice using ion-exchange method

为了分离苹果汁中的果酸,该文对离子交换法分离浓缩苹果汁中果酸的技术方法进行了系统研究,通过树脂分离果酸的静态和动态试验,对离子交换树脂进行了筛选优化,对树脂洗脱剂和洗脱条件进行了优选与优化,结果表明:D380树脂对果酸的交换吸附能力最强,其静态吸附量为30.73 mg/mL,动态吸附量可达60.24 mg/mL,静态交换吸附平衡时间为270 min,最佳吸附果汁浓度为11°Brix苹果汁;D380树脂对果酸的较佳动态吸附条件为:流速5 mL/min,温度20℃,果汁浓度11°Brix的苹果汁;较佳洗脱条件为:50%乙醇,温度25℃,流速6 mL/min,洗脱3次,洗脱剂与树脂比例2∶1(v/v)。

In order to separate fruit acid from apple juice, ion-exchange method was studied systematically. By static and dynamic fruit acid separation experiments, suitable ion-exchange resin type was selected, eluent and elution parameters was optimized. Results show that resin D380 has the highest absorption efficiency, and the static adsorbance is 30.73 mg/mL. The absorption balance time is 270 min. Dynamic adsorbance reaches 60.24 mg/mL. The optimal concentration of juice for absorption is 11°Brix. The optimum dynamic absorption parameters are as follows, flowing velocity 5 mL/min, temperature 20℃, juice concentration 11°Brix. The optimum elution conditions are as follows. 50%o ethanol as the eluent, temperature 25℃, flowing velocity 6 mL/min, 3-time elution, and the ratio of eluent to resin is 2 ： 1（v/v）.