MS培养基不同碳源和矮壮素浓度对马铃薯试管苗农艺性状的影响

Effect on the Growth of Potato Plantlets in MS Media with Different Carbon Sources and Concentration of Chlorocholine Chloride

选用马铃薯脱毒试管苗(大西洋,滇薯6号),MS培养基碳源(蔗糖,白砂糖)、MS培养基矮壮素浓度(0 mg·L^(-1)、100mg·L^(-1)、150mg·L^(-1)、200mg·L^(-1)、250mg·L^(-1)、300mg·L^(-1))进行3因素完全随机试验,观察处理后各试管苗生长的农艺性状。结果表明:以白砂糖作为碳源的MS培养基比以蔗糖作为碳源的MS培养基有利于大西洋和滇薯6号脱毒试管苗生长。矮壮素浓度为200mg·L^(-1)对马铃薯脱毒试管苗的壮苗效果较好。

A complete random test was conducted by a 3-factor experiment that virus-flee plandets of different varieties of potato （Atlantic and Dianshu No.6 ） were cultured on the MS media by used in which the sucrose or white crystal sugar was used as carbon source and chlorocholine chloride at 0mg·L^-1l,100mg·L^-1, 150mg·L^-1, 200 mg·L^-11, 250mg·L^-1, and 300mg·L^-1 was contained respectively. We have comparatively studied the agronomical characters of the virus-free plantlets of potato in different treatments. The results showed that the growth of virus-free plandets of Atlantic and Dianshu No.6 at the MS media with white sugar as carbon source was better than that on the MS media with sucrose as carbon source;And chlorocholine chloride concentration of 200mg·L^-1 was most suitable for the growth of Virus-Free Plandets of potato.