人卵巢癌细胞微观形态的AFM观察

Morphological Observation on Human Ovary Carcinoma Cells by AFM

目的:探讨原子力显微镜(AFM)在人卵巢癌细胞微观形貌表征方面的应用。方法:应用原子力显微镜分别观察培养的高低转移的人卵巢癌细胞及其周围纤连蛋白原纤维和经过紫杉醇药物处理后的细胞的微观形态,进一步对正常的卵巢癌细胞组织和经过紫杉醇药物处理后的卵巢癌细胞组织经过超声波处理后组织的微观结构进行AFM成像观察。结果:高转移特性的卵巢癌细胞周围的纤维少而短;而低转移特性的卵巢癌细胞周围的纤维多且长。经过紫杉醇药物处理后的细胞的形态发生了变化,结构呈现不规则状,且与基底没有纤维连接。结论:不同类型的卵巢癌细胞受其生物功能的影响在基底上的形态不同。药物处理后的癌细胞微观组织发生了变化,与其核溶,核碎和核解的生理变化过程相符。正常的卵巢癌细胞组织结构之间的黏附力较小,故超声波处理后呈现分散的分布。经过紫杉醇药物处理后的卵巢癌细胞组织结构之间的黏附力较大,超声波处理后分布在基底上的结构较紧凑。

Objective： To discuss the application of Atomic Force Microscope （AFM） in the observation of topographies of human ovary carcinoma cells and relations between microstructures of human ovary carcinoma cells and its biological functions. Methods： AFM was utilized to image the cell microstructures. AFM contact mode was used in the imaging process. Samples included three kinds of cells： normal human ovary carcinoma cells, cells dealt by medication and cells handled by the ultrasonic approach. Topographies of two kinds of human ovary carcinoma cells （with high transferring ability and low transferring ability） and its surrounding fibronectin fibrils and cells dealt with Paclitaxel were observed. Microstructures of human ovary carcinoma cells dealt with and without medication were also imaged after ultrasonic handling. Results： Number of fibrils around cells with high transferring ability was less and their length was shorter. No connection of these fibrils with other organs can be found. Number of fibrils around cells with low transferring ability was more and their length was longer. These fibrils were found to be connected with surrounding cells or organs. Cells dealt by Paclitaxel exhibited variations in topographies. The irregular shape was found： the convex structure in the middle of the cell and sucker-shape structures at each end of the cell were no longer found. The surface of the cell was more flat than normal cells. No fibronectin fibrils were connected to the substrate and other organs. Topographies of cells dealt with ultrasonic displayed scattering distributing on the substrate. Human ovary carcinoma cells handled by Paclitaxel and ultrasonic displayed collecting distributing on the substrate. Conclusions： Different types of cells and whether the cells were dealt with medication determined the microstructure properties of the human ovary carcinoma cells on the substrate. Fibrils were very important for biological functions of different cells in the organism： with shorter and longer fibrils around the cells for high transferring ability cells and low transferring ability cells, respectively. Because of the biological processes of karyon dissolving, karyon broken and karyon dissolution, the cells dealt by Paclitaxel were changed from the view of the microstructures. They agreed well each other. The distributing behaviors of cells on the substrate after ultrasonic handling showed that the adhesion forces between organs within normal human ovary carcinoma cells were less. The adhesion forces between organs within human ovary carcinoma cells handled by Paclitaxel were larger. These features agreed well with the biological variation process of the cells.