补肾清肺方对小鼠支气管哮喘模型的影响

Effect of Bushenqingfei Decoction on Airway Inflammation and Cytokine Production in a Mouse Asthmatic Model

目的:探讨中药复方补肾清肺方对小鼠哮喘模型气道炎症及细胞因子的影响。方法:40只BALB/c小鼠随机分为正常对照组(A组)、哮喘模型组(B组)、补肾清肺方低剂量组(C组)和补肾清肺方高剂量组(D组)。B、C、D组采用卵蛋白(OVA)腹腔注射致敏与雾化吸入激发制作哮喘模型。于OVA激发结束后24h收集支气管肺泡灌洗液(BALF)计数炎性细胞总数及嗜酸性粒细胞(EOS)数目,并测定BALF上清液中白细胞介素-4(IL-4)和干扰素-γ(IFN-γ)水平变化。结果:补肾清肺方的干预治疗能显著降低小鼠BALF中炎性细胞总数及EOS数量;BALF上清液中IFN-γ水平明显升高,IL-4水平显著下降。D、C组与A组、B组有显著性差异(P<0.05),C组与D组结果亦存在显著性差异(P<0.05)。结论:补肾清肺方能明显降低哮喘小鼠BALF中炎性细胞数量,影响细胞因子水平变化,从而改善哮喘气道炎症。

Objective： To explore the effect of Bushenqingfei decoction on the airway inflammation and the cytokine production in asthmatic mice. Methods： Forty BALB/c mice were divided into four groups： control group （group A）, asthmatic model group （ group B）, low dose of Bushenqingfei decoction group （ group C） and high dose of Bushenqingfei decoction group （group D） . The mice in group B, group C and group D were prepared by injecting ovalbumin （OVA） into the abdominal cavity and inhalation of aerosol. The number of eosinophil（EOS ） and the levels of IL-4 and IFN-γ in the mouse bronchoalveolar lavage fluids （BALF） were detected. Results： Bushenqingfei decoction could significantly reduce the number of EOS and the lcvel of IL-4, and increase the level of IFN-γ in the BALF of asthmatic mice. Significant difference was found between group D or C and group A or B （P〈0.05）. Group C and D also showed signifificant difference（P〈0.05 ）. Conclusion： Bushenqingfei decoction can rcmarkably reduce the number of inflammation cells in the BALF, and influence the level of cytokines so as to improve airway inflammation in the padents with asthma.