益母草对大鼠模型重症胰腺炎DIC形成作用的研究

Effect of motherwort on the formation of disseminated intravascular coagulation in severe acute pancreatitis

目的 探讨益母革对大鼠模型重症急性胰腺炎DIC的作用.方法 Wistar大鼠75只,雌、雄不限,体重180～300 g.随机分成5组,分别给予不同的处理,每组再分为6,12,24 h 3个时间点,每个时间点分配5只大鼠活杀取血测定血清AMY以及抗凝血浆测定TF抗原、血浆抗凝血酶Ⅲ（AT-Ⅲ）、血浆凝血酶原片段1＋2（F1＋2）、血浆凝血酶原时间（PT）.结果 各时间点各组的淀粉酶比较有统计学意义（P＜0.05）,病理切片除假手术组呈炎性改变.各时间点TF、AT-Ⅲ、F1＋2比较有统计学意义（P＜0.05）,24 h各组间PT比较有统计学意义（P＜0.05）.结论 虽然益母草对大鼠模型SAP本身疾病不起主要作用,但它能干预其凝血过程,有效地阻止DIC的发生和发展.

Objective To study the effect of motherwort on pre - DIC in a severe acute panereatitis rat model. Methods A total of 75 Wistar rats whose body 180 - 300 g in weight were randomized into 5 groups and were treated by different regimen. The experimental rats were killed 6, 12 and 24 hours after the establishment of models. Serum AMY, antithrombin -Ⅲ, plasma prothrombin fragment, plasma prothrombin time and tissue factor were determined. The results were analyzed by using one - way ANOVA, while the pancreas were observed by light microscopy. Results There were significant difference in serum AMY, antithrombin -Ⅲ, plasma prothrombin fragment, plasma prothrombin time and tissue factor（P 〈 0.01 ） among all graups at 6, 12 and 24 hours. Conclusion Though motherwort alone cannot prevent the inflammation process in SAP rat, it can prement the occurrance and progression of DIC by intervening the coagulation process.