人早期胎盘绒毛运动神经诱向因子1及其受体的免疫组织化学定位

IMMUNOHISTOCHEMICAL LOCALIZATION OF MOTONEURONOTROPHIC FACTOR 1 AND ITS RECEPTOR IN EARLY HUMAN PLACENTAL VILLI

为了解人胎盘绒毛是否存在运动神经诱向因子１（ＭＮＴＦ１）及其可能的功能意义，本实验用ＭＮＴＦ１单克隆抗体及抗独特型单克隆抗体在人早期胎盘绒毛石蜡切片上进行免疫组织化学反应，对人早期胎盘绒毛的ＭＮＴＦ１及其受体进行定位。结果显示，胎盘绒毛的细胞滋养层细胞、合体滋养层细胞和基质细胞均呈ＭＮＴＦ１强免疫反应，ＭＮＴＦ１样免疫反应物质分布在胞质内，胞核为阴性。在相邻切片上，以上３种细胞同样显示为ＭＮＴＦ１受体免疫反应，ＭＮＴＦ１受体样免疫反应物质也分布在细胞质内，胞核无免疫反应。以上结果显示，人胎盘绒毛可能会产生ＭＮＴＦ１，而且其产生的ＭＮＴＦ１可能通过自分泌和旁分泌方式对胎盘绒毛起局部调节作用。

In order to study whether the early human placental villi can produce motoneuronotrophic factor 1 and its potential functional significance, the localization of MNTF1 and its receptor in early human placental villi were studied by immunohistochemical reaction on the paraffin sections of early human placentas with MNTF1 monoclonal antibody and its anti idiotypic monoclonal antibody. The results revealed that the cytotrophoblasts, syncytio trophoblasts and the stroma cells in placental villi were MNTF1 positive immmunoreaction, the positive substances were distributed in cell plasma with negative nuclei. On the sections adjacent to above section, the cytotrophoblasts, syncytiotrophoblasts and stroma cells also were of MNTF1 receptor positive immunoreaction, the positive reactive substances were distributed in cell plasma with negative nuclei. These results suggest that the early human placental villi can produce MNTF1, which may take part in the local functinal regulation of early human placental villi by autocrine or paracrine.