激活素A和抑制素A对人绒毛膜上皮癌细胞系的增殖及分泌孕酮的影响

Effects of activin A and inhibin A on the proliferation and progesterone secretion of human choriocarcinoma cell line Jar cells were studied.

通过比较两种肽类激素（激活素和抑制素）对细胞增殖与激素分泌的作用，了解它们作用方式的差异。以来源于人胎盘的绒毛膜上皮癌细胞系Ｊａｒ细胞为实验材料，利用ＭＴＴ法测定细胞增殖，利用放射免疫法测定培养液中的孕酮含量，研究了激活素Ａ和抑制素Ａ对Ｊａｒ细胞的增殖和孕酮分泌的影响。结果表明，激活素Ａ（１～１００μｇ／Ｌ）对细胞增殖有明显的促进作用，且呈现浓度依赖性，浓度为３０μｇ／Ｌ时作用最强。与之相对比，抑制素Ａ对Ｊａｒ细胞的增殖无显著作用。同时，激活素Ａ能刺激Ｊａｒ细胞分泌孕酮，最大作用浓度为１０μｇ／Ｌ。抑制素Ａ对Ｊａｒ细胞分泌孕酮则没有明显的影响，以上结果提示，激活素Ａ对人绒毛膜上皮癌细胞的增殖和激素分泌起着较明显的调节作用，而抑制素Ａ则未见直接作用。

Cell proliferation and progesterone content in culture medium were determined by MTT test and by radioimmunoassay respectively.Activin A could stimulate cell proliferation and the effect was in a dose dependent manner,while the concentration of 30μg/L was most effective.In contrast,inhibin A had no obvious effect on cell proliferation.Activin A could also stimulate the progesterone production,but inhibin A could not.The most effective concentration of activin A was 10μg/L for progesterone stimulation.The results indicate that activin A may play an important role in regulating the proliferation and progesterone secretion of human choriocarcinoma cell line,but the role of inhibin A is still obscure.