胎儿脐动脉血管平滑肌细胞贴块培养方法的改进

The improvement of culture human vascular smooth muscle from fetus umbilical artery by explant attached method

目的探讨胎儿脐动脉血管平滑肌细胞的培养方法。方法取无菌新鲜健康孕妇胎儿脐动脉,采用组织贴块法培养血管平滑肌细胞,并用α-actin肌动蛋白免疫组化方法对培养的细胞进行鉴定。结果运用此方法成功地培养出胎儿脐动脉血管平滑肌细胞,培养的细胞呈典型的"峰-谷"样生长特性,用抗平滑肌α-肌动蛋白(α-actin)的单克隆抗体做免疫组化检测,低倍镜下可见细胞普遍呈阳性反应,高倍镜下可见α-actin肌动蛋白在胞浆内均匀分布。结论此方法取材容易,操作简单,费用低廉,为人动脉血管平滑肌细胞的培养提供了一种实用、可靠的方法。

Objective To, explore the culture techniques of fetus umbilical artery vascular smooth muscle cells. Methods The human vascular smooth muscle cells were cultivated using the fetus umbilical artery by the explant attached method in vitro, and identitfed by immunohistochemical methods. Results The fetus umbilical artery vascular smooth muscle cells were cultured successfully by using the method. The cells grew into the typical ＂hill-valley＂ characteristic They were identified by immunohistochemical methods of anti α- actin, it proved that all cells almost were vascular smooth muscle cells. Conclusion This method is easy, convenient and cheap. It provides a useful and reliable method for culture of human vascular smooth muscle cells.