扁穗牛鞭草种质遗传多样性的ISSR分析

Genetic diversity of Hemarthria compressa germplasm detected by inter-simple sequence repeat(ISSR)

用ISSR标记对来自中国西南地区（四川、重庆、贵州）的28份扁穗牛鞭草材料的遗传多样性进行了检测.从96个ISSR引物中共筛选出13个多态性明显、反应稳定的引物.28份材料的DNA共扩增出129条谱带,平均每个引物扩增出9.9条带,多态性条带比率达84.2%.材料间遗传相似系数为0.466～0.980,表现出丰富的遗传多样性.通过聚类分析和主成分分析,将28份扁穗牛鞭草分为两大类,同一地区的扁穗牛鞭草品种（系）基本聚在同一类,呈现出一定的地域性分布规律.

The inter-simple sequence repeat （ISSR） method was applied to detect genetic variation of 28 Hemarthria compressa accessions from Southwest China （Sichuan province, Chongqing city and Guizhou province）. Thirteen primers were selected from 96 ISSR primers, and 129 DNA fragments were amplified from 28 samples. Of these, 110 fragments （84.2%） were polymorphic and the average number of DNA bands was 9.9 per primer. The genetic similarity among all accessions ranged from 0. 466 to 0. 980, which indicated that the genetic diversity was comparatively rich among the accessions tested. UPGMA clusters based on genetic similarity and principle component analysis （PCoA） based on band patterns divided the accessions into two groups corresponding to their geographical sources. The application of ISSR analysis for cultivars or strain identification and classification of H. compressa are also discussed.