摘要
本研究在建立大鼠睾丸支持细胞体外培养方法的基础上，观察了不同浓度的硫丹、氯化镉对睾丸支持细胞的毒作用，发现它们对大鼠睾丸支持细胞均有毒作用，且有良好的时间效应和剂量效应关系。另外还研究了皮下注射２０μｍｏｌ／ｋｇ的氯化镉，２４ｈ后对所分离培养支持细胞存活的影响，发现体内给予氯化镉后，大鼠的支持细胞在体外培养４ｈ后存活率明显低于对照组（Ｐ＜０．０５）。随着培养时间的延长，其存活率差距逐渐增大，说明氯化镉可以透过血睾屏障到达支持细胞。本研究表明大鼠睾丸支持细胞体外测试系统具有快速、简便、终点明确、良好体内外一致性等优点，可用于雄性生殖毒物的筛试和毒作用机理的评价。
The primary culture of rat Sertoli cells was used in this study to assay the toxicity of diethylstibestrol,endosulfan and cadmium chloride on rat Sertoli cells.Sertoli cells were isolated from testes of 28-day-old rats and cultured in the presence of various conventrations of these toxicants for 24,48,96hr.We found that diethylstibestrol,endosulfan and cadmium chloride decreasing the survival rate of Sertoli cells with relevant dose and time-effect relationship.The study also found that the viability of Sertoli cells from cadmium chloride-exposed rats was significantly decreased (P<0.05).This study supported that Sertoli cells could be the target cell of cadmium chloride in agreement with the study in vivo.This study showed that the primary culture of Sertoli cells was a rather stable and sensitive method for the investigation of potential testicular toxicants.It may be used in the screening of testicular toxicants and the study of toxic mechanism.
出处
《癌变．畸变．突变》
CAS
CSCD
1997年第1期31-35,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
睾丸支持细胞
己烯雌酚
氯化镉
毒性
生殖毒性
Sertoli cells
Culture in Vitro
Toxicity
Diethylstibestrol
Endosulfan
Cadmium Choride
