摘要
目的:建立高效液相色谱-蒸发光散射检测(HPLC-ELSD)法同时测定柴胡中柴胡皂苷 a、c、d、f 含量。方法:采用Phenomenex C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相进行梯度洗脱(0 min:30:70,40 min:50:50,50 min:50:50),流速1.00 mL·min^(-1),Alltech ELSD-2000ES 检测器,漂移管温度:108℃,气体:空气,流速:3.0 L·min^(-1),柱温:室温。结果:柴胡皂苷 a、c、d、f 分别在0.18～8.84,0.17～8.28,0.21～10.40,0.16～8.20μg的范围内线性关系良好(r 分别为0.9997,0.9999,0.9997,0.9997);平均回收率(n=9)分别为103.3%,99.26%,102.0%,101.7%。结论:本法简便、准确,重复性好,可作为柴胡药材质量检测的方法。
Objective：To develop an HPLC -ELSD method for simultaneous determination of saikosaponins a, c, d,f in Radix Bupleuri. Methods：The analysis was performed on a Phenomenex C18 column （4. 6 mm × 250 mm, 5 μm） at the room temperature with a gradient program of acetonitrile -water （0 min：30：70,40 min：50：50,50 min：50： 50） as the mobile phase and an Evaporative Light- Scattering Detector （Alhech ELSD-2000ES） as the detector. The drift tube temperature was 108℃, the gas flow rate was 3.0 L · min^ - 1. Results： Saikosaponins a, c, d,f were linear over the range of 0. 18 - 8.84,0. 17 - 8.28,0. 21 - 10.40,0. 16 - 8.20 μg （ r was 0. 9997, 0. 9999,0. 9997,0. 9997, respectively）. The average recoveries （ n = 9 ） were 103.3% , 99. 26% , 102. 0% , 101.7 % for saikosaponins a, c, d, f, respectively. Conclusion： This method is simple, sensitive, reliable and reproducible. It can be used for the quality control of Radix Bupleuri.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第8期1150-1153,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家科技基础条件平台工作中药材质量标准项目(2003DIA7J031)
科技部国家科技攻关计划创新药物与中药现代化项目(2001BA701A41)
