獐牙菜属及近缘植物中4种有效成分的HPLC定量分析

Quantitative HPLC analysis of four effective components in Swertia genus and related plants

目的:比较獐牙菜属及近缘植物中獐牙菜苦苷、龙胆苦苷、芒果苷、齐墩果酸的含量,为资源利用提供依据。方法:獐牙菜苦苷、龙胆苦苷、芒果苷色谱条件:ZORBAX SB-C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相甲醇-水(含0.1%磷酸)的比例在0→30 min 内由20:80线性变化至30:70,检测波长240 nm,流速0.60 mL·min^(-1),柱温30℃。齐墩果酸色谱条件:ZOR-BAX SB-C_(18)色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-水(含0.1%磷酸)(75:25),检测波长210 nm;流速1.0 mL·min^(-1);柱温40℃。结果:獐牙菜苦苷、龙胆苦苷、芒果苷及齐墩果酸的线性范围为0.155～19.0μg(r=0.9999),0.0186～1.24μg(r=0.9999),0.00680～1.36μg(r=0.9998),0.150～3.90μg(r=0.9996),回收率分别为101.2%(RSD=1.6%),99.4%(RSD=1.2%),102.6%(RSD=1.5%),99.3%(RSD=1.8%)。结论:本实验说明在民间作为獐牙菜使用的植物中都含有4种有效成分,但种间差异较大。

Objective： To analyze and compare the content of swertiamarin, gentiopicroside, mangiferin, oleanolic acid in 14 species of Swertia and its related plants and to supply a basis for the resources exploitation. Methods： The ZORBAX SB -C18 column （4. 6 mm 150 mm, 5 μm）was used. The mobile phase of swertiamarin, gentiopicroside, mangiferin consisted of CH3OH -H2O （0. 1% H3PO4 ） ,gradient elution ：0→30 min from 20：80 to 30：70 ;the flow rate was 0. 60 mL· min^-1, and the detection wavelength was 240 nm. The mobile phase of oleanolic acid consisted of CH3 CN - H2 O （0. 1% H3 PO4 ） （75：25 ） , the flow rate was 1.0 mL·min^-1, and the detection wavelength was 210 nm. Results：The linear ranges of swertiamarin, gentiopicroside, mangiferin and oleanolic acid were 0. 155 ～ 19.0 μg（r =0. 9999） ,0. 0186 - 1.24 μg（r =0. 9999） ,0. 00680 - 1.36 μg（r =0. 9998） ,0. 150 -3.90μg （r =0. 9996） respectively. The average recoveries were 101.2% （RSD = 1.6% ） ,99. 4% （RSD = 1.2% ）, 102.6% （RSD = 1.5% ） ,99.3% （RSD = 1.8% ） respectively. Conclusion ：This experiment shows that these plants used for Zhangyacai in folk medicine contain four effective components, but the difference is notable.