反相高效液相色谱法测定岩黄连药材中脱氢卡维汀的含量

RP-HPLC determination of dehydrocavidine in Corydalis saxicola Bunting

目的:建立反相高效液相色谱法测定岩黄连药材中脱氢卡维汀的含量,并用该方法测定不同采收时间、不同部位岩黄连药材中脱氢卡维汀的含量。方法:采用 Phenomenex Gemini-C_(18)色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.02mol·L^(-1)磷酸二氢钾溶液(74:26),二元等度洗脱系统;流速为1.0 mL·min^(-1);检测波长346 nm;柱温为室温。结果:脱氢卡维汀浓度在8.4～84μg·mL^(-1)范围内线性良好,回归方程为 Y=8.550×10~4X-1019(r=0.9998),低、中、高3个加入量的平均回收率分别为101.3%,102.0%,105.3%;RSD 分别为2.8%,2.3%,2.4%;不同采收时间岩黄连全草中脱氢卡维汀的含量为1.72%～2.07%,在根、茎、叶、花中的含量分布为花>根>叶>茎。结论:本方法简便、准确,重复性好,适用于岩黄连药材的质量控制研究。

Objective：To establish a quantitative analysis method for dehydrocavidine in Corydalis saxicola Bunting using RP - HPLC. Methods： A Phenomenex Gemini - C 18 column （ 250 mm × 4.6 mm, 5μm） was used with acetonitrile - water （ containing 0. 02 mol ·L^-1 postassium dihydrogen phosphate） （ 74： 26 ） as the mobile phase at a flow rate of 1.0 mL· min^ -1, and detected at 346 nm. Results： The calibration curve was linear in the range of 8.4 -84μg· mL^-1. The average recoveries were 101.3% ,102.0% ,105.3% and RSD were 2. 8% ,2.3% ,2.4%, respectively. Conclusion：This method is rapid, accurate and reproducible for the quantitative analysis of dehydrocavidine in C. saxicola Bunting and can be used for the quality control of the crude drug.