黑骨藤根挥发性成分研究

Determination of chemical constituents of essential oil from root of Periploca forrestii Schltr

目的:研究黑骨藤根挥发性成分。方法:利用水蒸气蒸馏法提取黑骨藤根挥发性成分,用 GC-MS 进行分离测定,色谱柱为 SE-30弹性石英毛细管柱(30 m×0.25 mm×0.25μm),柱温50℃,保持2 min,然后以4℃·min^(-1)升温至230℃,保持5 min。载气为高纯度氦气99.999%;载气流量1.0 mL·min^(-1);分流比40:1。结合计算机检索技术对分离的化合物进行结构鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果:分离出26个成分,确定了21个化合物,占挥发油总量的95.61%。黑骨藤根挥发性成分大于1%的化学成分有5种,分别为:薁1.177%,α-雪松醇1.247%,芳樟醇1.248%,甲基萎叶酚2.297%,反式茴香脑83.013%。结论:本文对黑骨藤根挥发性成分的研究,为该类植物的进一步研究开发提供必要的试验数据。

Objective： To study the composition of essential oil root of Periploca forrestii Schhr. Methods： The oil was obtained by steam distillation. The chemical compositions were separated and identified by GC - MS equipped with a elastic quartz capillary column - SE - 30 （30m×0.25mm×0.25μm）. The oven temperature program was 50 ℃ for 2 min,increased at 4 ℃ ·min^-1 to 230 ℃ for 5 min. The carrier gas was helium （99. 999% ） with a flow rate of 1.0 mL·min^ - 1. The split ratio was 40： 1. The relative percentage of the oil constituents was calculated from the GC peak areas. Results：Twenty - six components were detected in the essential oil of Periplocaforrestii Schhr. ; of which twenty - one compounds representing 95.61% of the oil were characterized. The major constituents of essential oil of root of Periploca forrestii Schltr. which were above 1% were azulene （ 1. 177% ）, α - cedrol （ 1. 247% ） ,linalool （ 1. 248% ） ,methyl chavicol（2. 297% ） ,E - anethole （83. 013% ）. Conclusion：The determination of chemical constituents of essential oil from root of Periplocaforrestii Schhr. could give some useful information for the further researching of Periploca forrestii Schhr..