摘要
对19例全身型重症肌无力(MG)患者周围血B细胞分化能力进行了研究,并观察重组白细胞介素2(rIL-2)及T细胞亚群对其影响。方法分离外周血纯B细胞,体外与电鳗乙酰胆碱受体(AChR)一起培养,并分别加入去CD4+T细胞,去CD8+T细胞及rIL-2。用酶联免疫-生物素法检测其上清液的乙酰胆碱受体抗体(AChR-ab)。同时以10例健康人为对照组。结果去CD8+T细胞后AChR-ab的产生无明显增加;去CD4+T细胞加入rIL-2后AChR-ab产生减少。结论提示rIL-2对MG患者有潜在治疗价值。
Objective The differentiation of B lymphocytes from the peripheral blood were studied in 19 patients with myasthenia gravis (MG) and 10 healthy volunteers. The purpose of this research was to investigate the effects of recombinant interleukin 2(rIL 2) and T lymphocyte subsets on differentiation of B cells from the peripheral blood in MG patients. Mathods The amount of acetylcholine receptor autoantibody (AChR ab) which the B lymphocytes producted after eight days culture in vitro in the presence of AChR and T lymphocyte subsets was determined. Results The results showed that there was no significantly increase in AChR ab production by removed CD8 + T cell groups and the production of AChR ab was decreased when rIL 2 was added in the presence of CD8 + T cell groups. Conclusion It was suggested that rIL 2 might have certain potential value in the treatment of MG.
出处
《中华神经科杂志》
CAS
CSCD
1997年第2期74-76,共3页
Chinese Journal of Neurology
基金
国家自然科学基金