HPLC法测定莲芝消炎片中穿心莲内酯和脱水穿心莲内酯的含量

Determination of Lianzhi Xiaoyan Pill by HPLC

建立莲芝消炎片中脱水穿心莲内酯、穿心莲内酯含量的HPLC测定方法.色谱柱为SUNTEKKromasil C18柱;流动相为甲醇-水（60∶40）,流速为0.8 mL·min^-1,检测波长为250 nm.线性范围为脱水穿心莲内酯0.8000～2.240μg,r=0.9999,穿心莲内酯0.1011～0.2831μg,r=0.9995,平均回收率脱水穿心莲内酯为98.99%,RSD为1.4%,穿心莲内酯为101.48%,RSD为1.5%.方法准确可靠,简单易行,适用于莲芝消炎片中脱水穿心莲内酯、穿心莲内酯的含量测定.

A HPLC method for the determination of Lianzhi Xiaoyan Pill was established. A SUNTEK Kromasil C18 column was used with methanol-water （60 ： 40） as mobile phase. The flow rate was 0. 8 mL · min^-1 and detection wavelength was 250 nm, The linear range for deoxyandrographolide was 0. 8000- 2. 240μg （r=0. 9999） and those for andrographolide was 0. 1011 -0. 2831μg （r= 0. 9995） . The average recovery and RSD for deoxyandrographolide were 98.99 % and 1.4 %, and those for andrographolide were 101.48% and 1.5% respectively. The method was accurate, reliable, simple and feasible, it could be used for the content determination of Lianzhi Xiaoyan Pill.