分光光度法测定-超声波提取植物韭菜中叶绿素含量的研究

利用超声波浸提了植物韭菜中的叶绿素,探讨了一种超声浸提植物叶片中叶绿素的新方法。通过对叶绿素浸提条件的实验,根据超声浸提与直接浸提结果的比较可以看出,超声波浸提的浸提剂减少,时间明显减少,一次浸提仅为30min。当韭菜含水率为20%～30%时,测得其吸光度为0.707。浸提4次时,基本浸提完全,结果较为理想,比直接浸提10h时的吸光度高约13%。

A new method of using ultrasonic wave to endosmosis chlorophyll from leek was studied. By comparing the results of ultrasonic wave endosmosis and directing extraction on endosmosis experimental condition, the result showed that ultrasonic wave endosmosis was better than directing extraction which had use less extractant but gain higher concentration. The ultrasonic wave endosmosis time was only 30min. When the moisture content of leek was 20% -30%, 4 times extraction was the best, and the absorbency was 0.707 which was 13% higher than the directing extraction.