摘要
为了评价恩诺沙星(enrofloxacin)对土壤微生物群落的影响,借助BIOLOG检测法比较了不同浓度恩诺沙星影响下的土壤微生物的群落特征。结果表明,各添加药物组与空白对照组的土壤微生物群落代谢多样性差异显著(P<0.05),空白对照组土壤微生物在BIOLOG微平板上的平均每孔颜色变化率(AWCD)和微生物代谢多样性指数(丰富度和多样性)显著高于添加药物组;药物显著影响微生物群落对BIOLOG微平板中碳源的利用能力,较低浓度的恩诺沙星(0.01μg/g)就显著影响了土壤微生物群落对α-Ketobutyric Acid、L-Phenylalanine、1-Erythritol、D-Xylose等碳源的利用代谢能力(P<0.05),且土壤微生物群落利用各类碳源的能力随药物浓度加大而降低。由此可见,恩诺沙星在土壤中残留时对土壤微生物的影响不容忽视。
Functional diversity of soil microbial communities was examined by microbial metabolic diversity (BIOLOG) assays to evaluate the effects of enrofloxacin with different concentrations on soil microbial communities. The results showed that soil microbial metabolic diversity in CK was significantly higher than that in the soils in which the enrofloxacin was added. In BIOLOG assays, the average well colour development (AWCD) and the richness and diversity indices indicated the differences of microbial metabolic diversity. It also indicated that the enrofloxacin significantly affected the sole-carbonsource metabolic ability of soil microbial communities, While low concentration of enrofloxacin (0.01 μg/g) significantly affected the metabolic ability of sole-carbon-source, such as α-Ketobutyric Acid, L-Phenylalanine, 1-Erythritol, D-Xylose and so on. The effects of enrofloxacin on functional diversity of soil microbial communities were enhanced with the increasing enrofloxacin concentration. Therefore, the potential effects of enrofloxacin on the soil microbial communities warrant further studies.
出处
《生态学报》
CAS
CSCD
北大核心
2007年第8期3400-3406,共7页
Acta Ecologica Sinica
基金
国家自然科学基金重点资助项目(30130140)~~