天花粉蛋白对荷人结肠癌SW-1116裸鼠的体内实验研究

Study on The Anticolon Carcinoma SW-1116 Effects of TCS in Vivo

目的：研究天花粉蛋白（TCS>）体内抗结肠癌作用。方法：将人结肠癌SW－1116移植于Swiss-Df品系8周龄裸小鼠肾包膜下，次日以不同剂量TCS注射入探小鼠腹膜腔内，以丝裂霉素（MMC）为阳性对照，生理盐水（NS）为阴性对照，每日给药一次，连续五日，观察天花粉蛋白对移植瘤体内抑制情况。结果：TCS剂量为0.75mg/kg组对移植瘤SW－1116的抑癌率为41.2％（P＜0．001），较1.0mg／kgMMC组（抑癌率31.28％）为佳；0.5mg/kgTCS组抑癌率为27.50％（P＜0.05），远小于TCS对小鼠的半数致死量（13．4mg/kg）。结论：TCS对结肠癌SW－1116裸鼠有体内抑癌作用。

Purpose :To study the effects of anticolon carcinoma (SW-1116) of Trichosancin (TCS) in vivo. Methed: SW-1116 human colon carcinoma were transplanted in subrenal capsules of eight-week Swiss-Df strain nude mice, the different dosages of TCS were injected into nude mice peritoneal cavities next day after transplantation for 5 days, control groups were treated with normal saline (NS, negative control) and mitomycin-c (MMC, positive control), then the tumor growth were observed. Results: The inhibition rate oi TCS in 0.75mg/kg group on the transplanted tumor was 41. 20 %, superior to that of 1. 0mg/kg MMC group (31. 28 % ). The effecti ve dosages of TCS was 0. 05mg/kg (inhibition rate 27. 5 %, P＜0. 05), much lower than LD50(13. 4mg/kg) of mice. Conclusion:This experiment confirmed that the TCS have the anticoloncarcinoma SW-1116 effects in vivo.