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RNA扩增的研究进展 被引量：3

Progress of RNA amplification
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摘要 RNA扩增是伴随着基因芯片技术的应用而发展起来的一项技术,但不仅仅应用于芯片杂交。文章对RNA扩增的技术方法进行了综述,重点介绍了mRNA的线性扩增、指数扩增以及两者相结合的扩增方法以及优缺点,对microRNA的扩增方法也作了介绍,并对将来的发展作了展望。 The technology of RNA amplification is developing with the application of gene chip and it is not only adapted for chip hybridization, but also applied in other purposes as well. The methodologies of RNA amplification were reviewed, focused on linear, exponential and linear plus exponential amplifications. The amplification of microRNA was also introduced and a prospect of RNA amplification was made.
作者 李轶女 胡英考 张志芳 沈桂芳
机构地区 中国农业科学院生物技术研究所 首都师范大学生命科学学院
出处 《遗传》 CAS CSCD 北大核心 2007年第8期907-914,共8页 Hereditas（Beijing)
基金 国家自然科学基金项目(编号: 30670082)资助~~
关键词 RNA扩增 体外转录 聚合酶链式反应 基因芯片 小RNA RNA amplification in vitro transcription PCR microarray microRNA
分类号 Q78 [生物学—分子生物学]
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参考文献32

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同被引文献32

  • 1Richmond T, Somerville S. Chasing the dream: Plant EST mi- croarrays. Curr Opin Plant Bio12000; 3(2): 108-16.
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2007年 第8期

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