摘要
在鸡1号染色体上选取23个微卫星标记,利用东北农业大学鸡F2资源群体构建了遗传连锁图谱。选用369只F2个体用于基因型测定。结果表明23个微卫星位点除MCW0058为低度多态,其他位点均为中高度多态。构建的连锁图谱覆盖1号染色体全长,总共637.9cM。MCW0115和ROS0025标记顺序与EL图谱不同,但与WAU图谱一致。其他标记顺序与3大参考家系标记顺序一致,图谱总长和标记间距离大于3大参考家系。此连锁图谱的构建为数量性状位点(QTL)定位奠定了良好的基础。
Three hundred and sixty-nine F2 individuals produced from Northeast Agricultural University Resource Population (NEAURP) were genotyped by 23 fluorescent microsateUite markers on chromosome 1. The characterization of these microsatellites was moderate or high polymorphic except marker MCW0058 which was low polymorphic. The length of the sex averaged linkage map was 637.9 cM. The order of MCW0115 and ROS0025 disagreed with that of EL map, but consisted with that of W map. The intervals of markers were larger than those of three reference families.
出处
《遗传》
CAS
CSCD
北大核心
2007年第8期977-981,共5页
Hereditas(Beijing)
基金
教育部新世纪优秀人才支持计划项目(编号:NCET-04-0343)
国家自然科学基金重点项目(编号:30430510)
国家高技术研究发展计划项目(863计划)(编号:2006AA10A120)资助~~
关键词
鸡
F2资源群体
微卫星
连锁图谱
chicken
F2 resource population
microsatellites
genetic linkage map