HPLC测定人血浆中的塞克硝唑及其药物动力学参数

Determination of Secnidazole in human plasma and study on pharmacokinetics by HPLC

目的建立测定人血浆中塞克硝唑浓度的方法。方法血浆样品经10%三氯醋酸沉淀蛋白后,用HPLC法,以乙腈-0.05 mol.L-1枸橼酸缓冲液(pH4.0,18:82)为流动相,经Diamonsil-C18色谱柱分离,310 nm检测。结果塞克硝唑在1.0～60.0μg.ml-1与待测物和内标物峰面积比的线性关系良好,最低检测浓度为1.0μg.ml-1;低、中、高3个浓度的提取回收率为82.10%～84.69%,日内、日间RSD低于6.09%。结论所建方法准确、操作简单,可满足临床药物动力学研究的要求。

OBJECTIVE To establish a method for determination of Secnidazole in human serum. METHODS Serum protein was precipitated with trichloroacetic acid. The analysis was conducted with a Diamonsil - C18 （ 150 mm × 4. 6 mm,5 μm） column, and acetonitrile-0. 05 mol·L^-1 citric buffer（ pH4. 0,18 ： 82 ） was served as mobile phase with the detecting wavelengh at 310 nm . RESULTS The linear range of Secnidazole was 1.0 - 60. 0 μg·ml^-1 （ r = 0. 9990） and the average recoveries were 82. 10% - 84. 69 % at three different concentrations . The RSD of intra - and inter - day were 1.86% - 6. 09% . CONCLUSION This method is suitable for the pharmacokinetic study of Secnidazole .