核酸原位杂交检测尖锐湿疣的临床价值

THE CLINIC VALUE OF HYBRIDIZATION IN SITU IN THE DIAGNOSIS OF CONDYLOMA ACCUMINATION

目的提高尖锐湿疣的诊断正确率。方法选光镜下初诊尖锐湿疣病例１３例，临床怀疑尖锐湿疣而光镜下不典型者８例，进行尖锐湿疣乳头状瘤病毒（ＨＰＶ）６Ｂ／１１型核酸原位杂交检测。结果光镜下初诊尖锐湿疣的１３例中９例阳性，阳性率为６９％；光镜下不典型的８例中仅３例为阳性，阳性率为３８％。结论仅以临床表现诊断尖锐湿疣正确率低，病理组织学检查结合核酸原位杂交检测是确诊尖锐湿疣有价值的方法。

Objective To improve the diagnosed rate of condyloma accumination.Method 13 cases which were diagnosed as condyloma pathological and 8 cases which were diagnosed as condyloma clinicaly were detected by using DNA hybridization on situ.Result The HPV DNA sequences were positive in 9/13 condyloma accuminata which were typical pathological and 3/8 of the suspiciouse cases clinically.Conclusion [WT5BZ〗DNA hybridization in situ technique conbined with hystopathology would be of great value for the diagnosis of condyloma accumicatum.