升麻甙与5-O甲基维斯阿米醇甙对血管内皮细胞分泌细胞因子的影响

The effect of prim-O-glucosylcimifugin and 4'-O-β-D-glucosyl-5-O-methylvisamminol con on the secretion of cytokines of cardiac microvascular endothelial cells

目的:观察升麻甙与5-O甲基维斯阿米醇甙对氧化低密度脂蛋白(ox-LDL)致心脏微血管内皮细胞损伤,引起IL-6和TNF-α分泌的影响。方法:采用体外培养的乳鼠心脏微血管内皮细胞,测定其生长曲线及药物的最大无毒浓度后,经升麻甙(50、100、200μg/ml)与5-O甲基维斯阿米醇甙(50、100、200μg/ml)预处理24h,再与ox-LDL(MDA含量为3nmol/ml)共同孵育24h,用ELISA法和放免法分别检测细胞培养液中IL-6和TNF-α的水平。结果:升麻甙与5-O甲基维斯阿米醇甙在50、100、200μg/ml时能够明显抑制ox-LDL引起的内皮细胞IL-6、TNF-α分泌,且呈剂量依赖性。结论:升麻甙与5-O甲基维斯阿米醇甙能够抑制血管内皮细胞产生炎性细胞因子IL-6和TNF-α。

Object ： To investigate the effect of prim - O - glucosylcimifugin and 4′- O - β - D - glucosyl - 5 - O - methylvisamminol con on the secretion of IL -6 and TNF -α of cardiac microvascular endothelial cells induced by oxidized low density lipoprotein（ ox - LDL）. Methods： The growth curve of rat cardiac microvascular endothelial cells and the maximum avirulent concentration of prim - O - glucosylcimifugin and 4′-O- β- D- glucosyl- 5 -O- methylvisamminol con was detected. After pretreatment with prim- O- glucosylcimifugin（ 50,100,200 μg/ ml） and 4′- O - β - D - glucosyl - 5 - O - methylvisamminol con（ 50,100,200μg/ml） for 24 hours, the cardiac microvascular endothelial cells was incubated with ox -LDL for another 24 hours, then the level of IL- 6 ,TNF-α in supematant was detected by ELISA and RIA respectively. Results： The secretion of IL - 6 and TNF - α induced by ox - LDL was inhibited obviously by prim - O - glucosylcimifugin and 4′- O - β - D - glucosyl - 5 - O - methylvisamminol （50,100,200ug/ml） in a dose dependent way. Conclusion： The secretion of IL - 6 and TNF - α from cardiac microvascular endothelial cells can be inhibited by prim - O - glucosylcimifugin and 4′- O - β - D - glucosyl - 5 - O - methylvisamminol con, which indicated that the two drugs intervene inflammatory reaction of AS.