血塞通注射液对大鼠心肌肥厚及氧化应激的影响

Effects of Xuesaitong Injection(血塞通注射液) on myocardial hypertrophy and oxidative stress in rats

目的:研究血塞通注射液(Xuesaitong Injection,XST)对异丙肾上腺素致大鼠心肌肥厚的保护作用及对氧化应激的影响。方法:采用异丙肾上腺素(isoproterenol,ISO)5mg/kg·dsc连续7d。诱导大鼠心肌肥厚模型。大鼠随机分为NS对照组、ISO模型组、XST(25、50mg/kg)治疗组。测定全心重量指数(HW/BW),左心室重量指数(LVW/BW即LVI);采用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和丙二醛(MDA)的含量,超氧化物歧化酶(SOD)、谷胱苷肽过氧化物酶(GSH-Px)的活力以及血清中超氧化物歧化酶(SOD)活力、磷酸肌酸激酶(CK)和丙二醛(MDA)含量的变化。结果:ISO模型组大鼠的心脏重量指数明显增大,左心室Hyp、MDA含量增高,SOD、GSH-Px活力显著下降;血清MDA、CK水平明显升高,SOD活力明显下降。PNS能明显降低血清中CK的释放,降低血清和心肌中MDA水平及提高SOD活力,保护心肌组织GSH-Px酶活性,减少心肌组织胶原的含量,减轻心脏重量指数,抑制心肌肥厚。结论:XST对ISO所致大鼠心肌肥厚具有一定的保护作用,其机制可能与抗氧化作用有关。

Object： To investigate the antioxidative effects of Xuesaitong injection（XST） on myocardial hypertrophy induced by isoproterenol （ISO） in rats. Methods： Myocardial hypertrophy model of rats were induced by injection of ISO （5 mg/kg·d,sc） for 7d. Rats were rabdomly divided into NS control group , ISO model group and two XST groups （25,50 mg/kg）. Then, the myocardial indexes were measured;the hydroxyroline and malondialdehde （MDA） content of left ventricle,the activity of superoxide dismutase （SOD） and glutathione peroxidase （ GSH - Px） in left ventricle were determined ; the activity of SOD , the levels of CK, MDA in serum were measured by spectrophotometry respectively. Results： In ISO model group ,the myocardial indexes and the content of hydroxyproline and MDA in left ventricle markedly increased; the activilty of SOD and GSH- Px in left ventricle were decreased; the content of MDA and CK in serum markedly increased; the activihy of SOD in serum were decreased. Treatment with XST significantly reduced the myocardial indexes and the hydroxyproline and MDA content, increased the activity of SOD and GSH- Px in heart tissue; increased the activity of SOD, significantly reduced the MDA and CK content in serum. Conclusion： XST was shown to prevent remodeling of myocardial hypertrophy induced by ISO in rats ; The effect of XST on anti -hypertrophy was associated with inhibiting oxidative response.