红芪多糖HPS-3体外诱导人胃癌MGC-803细胞凋亡的研究

Study of apoptosis induced by Hedysari Polysaccharide HPS-3 on human gastric cancer cell line MGC-803 in vitro

目的:研究红芪多糖均一组分HPS-3体外对人胃腺癌MGC-803细胞增殖及凋亡的作用。方法:应用MTT法、显微荧光术和流式细胞术测定HPS-3对MGC-803细胞的增殖、细胞周期和诱导凋亡的影响,应用流式细胞术测定对Bc1-2和Bax蛋白表达的影响。结果:HPS-3对MGC-803细胞具有抑制增殖、G2/M期阻滞和诱导凋亡作用;HPS-3降低bcl-2蛋白表达,对Bax蛋白表达无明显作用。结论:HPS-3可通过抑制人胃腺癌MGC-803细胞的生长增殖、G2/M期阻滞、诱导细胞凋亡,降低bcl-2蛋白等作用机制发挥肿瘤作用。

Object： To study the effect of Hedysari polysaccharide HPS - 3 on the proliferation and apoptosis of human gastric cancer cell line MGC -803 in vitro. Methods： MTT assay,flow cytometry and fluorescence microscopy were used to examine the effects of HPS -3 in anti - proliferation, cell cycle arresting and apoptosis induction. Flow cytometry were used to detect the effects on protein expression of Bcl - 2 and Bax. Results： HPS -3 could cause apparent inhibition on cell growth ,induce G2/M phase arrest and apoptosis on MGC -803 cells. HPS - 3 could down - regulate bcl - 2 protein expression, but Bax protein expression showed unchanged. Conclusion： HPS - 3 shows its anti - tumor effects by way of inhibiting the cell growth, arresting the G2/M phase, inducing apoptosis, down - regulating bcl - 2 protein expression.