含γ-亚麻酸的高产脂菌种的诱变选育

Mutation breeding of high-yield oil producing strain containing γ-linolenic acid

以被孢霉为出发菌种,经常规紫外线诱变,利用含氯化锂的低温或高糖诱变培养基进行初筛,再经摇瓶发酵和测定相关性能指标,筛选到一株突变菌株ZG17。菌株ZG17具有良好的吸收和利用葡萄糖并将其转化为油脂的能力,其油脂含量达到69%,γ-亚麻酸含量为4.8%。

Motierella sp. was mutagenized with UV and screened by selecting mutants resisting low temperature or high concentration of glucose in culture medium containing LiCI. The result indicated that a mutant ZG17 had good capability of utilizing glucose and converting glucose to single cell oil. The oil content in ZG17 mycelium was up to 69% and the content of γ-linolenic acid in oil from ZG17 mycelium was 4.8%.