亮氨酸氨基肽酶基因多态性与原发性高血压相关性研究

Association Between A-LAP Gene Polymorphism and Essential Hypertension

目的探讨脂细胞衍生亮氨酸氨基肽酶(A-LAP)基因的多态性与原发性高血压的相关性。方法采用关联分析,严格按照标准对湖南地区汉族随机选取,其中原发性高血压患者135例,正常对照135例。应用聚合酶链式反应-限制性片段长度多态性分析(PCR-RFLP)对两组对象的A-LAP的828A>G多态性进行分析。结果AA,GG,AG三种基因型分布频率在正常对照组分别为61.5%、1.5%、37.0%,在高血压病组中分别为48.1%、5.9%、45.9%,两组间基因型频率比较χ2=7.075,df=2,P=0.029,差异有显著性;A和G等位基因分布频率在正常对照组中分别为80.0%、20.0%,在高血压病组中分别71.1%、28.9%,A和G等位基因频率比较χ2=5.775,df=1,P=0.016,差异有显著性。结论多基因联合分析显示,高血压病组A-LAP等位基因G的频率高于等位基因A,828A>G对发生原发性高血压的发生相对危险有协同作用。

Objective To study the relationship between A- LAP gene A828G polymorphism and essential hypertension. Method The A- LAP gene A828G polymorphism was detected in 13.5 patients with essential hypertension and 135 controls by PCR - RFLP. Results The frequencies of AA, GG and AG genotype were 61.5 %, 1.5 %, and 37.0 % in the control group and 48.1%, .5.9 %, and 4.5.9 % in the hypertensive group respectively, significant difference of the allele distributions frequencies in these 2 groups was evident （χ^2 = 7.075, df=2, P= 0. 029）. A and G allele distribution frequencies in these 2 groups were 80.0 % and 20.0 % in the control group, 71.1% and 28.9 % in the hypertensive group; again significant difference of A and G distributions frequencies in these 2 groups was evident （χ^2 = 5.775, df = 1, P = 0. 016）. Conclusions There is a relationship between the A - LAP gene A828G polymorphism and hypertension. G allele may be a risk factor of hypertension.