华南汉族健康者凝血因子VII R353Q基因型的分布

Distribution of Coagulation Factor VII Gene R353Q Polymorphisms in Han Chinese in the South of China

目的观察凝血因子VII(coagulationf actor VII,FVII)R353Q基因多态性在华南汉族健康者人群中的分布。方法提取华南汉族60名正常健康者基因组DNA,应用多聚酶链反应(PCR)技术和限制性内切酶片段长度多态性技术检测上述人群的FVII R353Q基因型。结果华南汉族人群FVII基因R353Q具3种基因型(RR,RQ,QQ),FVII等位基因R、Q基因频率在人群中分别为90.9%,9.1%。基因型频率符合Hardy-Weinberg平衡定律。结论华南汉族健康人群凝血因子VII基因R353Q多态具3种基因型(RR,RQ,QQ),R等位基因携带频率明显高于Q等位基因。

Objective To study the distribution of coagulation factor VII gene R353Q polymorphisms in Chinese Han population in the south of China, Methods Genotypes R353Q polymorphisms of FVII were typed in 60 healthy subjects in the south of China by polymerase chain reaction - restriction fragment length polymorphism. Results Three gene types （RR, RQ, and QQ） of R353Q polymorphisms were seen, FVII allelic frequencies of R and Q were 90.9% and 9.1% in the 60 healthy subjects respectively. Genotype distribution was in accordance with Hardy - Weinberg equilibrium. Conclusion There are three types R353Q polymorphisms of the FVII gene in Han Chinese in the southern of China.