湖南省未接受抗病毒治疗的HIV-1毒株耐药基因变异研究

Longitudinal Survey of Antiretroviral Drug Resistance Among Untreated HIV-1 Infection Individuals in Hunan Province

目的研究湖南省HIV感染者/AIDS患者的耐药情况。为开展大规模抗病毒药物治疗制定计划和方案时提供科学依据。方法对51名HIV感染者/AIDS患者进行CD4绝对计数、血浆病毒载量测定、基因型耐药性检测,并进行分析。结果发现1份样品存在蛋白酶抑制剂(PIs)主要耐药相关突变V32L,N88D(2.13%),3份存在次要耐药相关突变,分别为A71T(4.26%)、A71V(2.13%),3份对核苷类逆转录酶抑制剂(NRTIs)耐药突变L210M(2.13%)、L210F(4.26%),4份对非核苷类逆转录酶抑制剂(NNRTIs)耐药突变V179D(6.38%)、V108A(2.13%)。结论湖南省未接受抗病毒药物治疗的HIV感染者/AIDS患者中对现行的抗病毒治疗方案药物耐药水平仍然很低,但是应该加强对HIV感染者/AIDS患者体内HIV-1变异的监测。

Objective To study the antiretroviral drug resistance incidence among untreated HIV- 1 infection individuals in Hunan Province, and to provide scientific basis for regimens design of antiretroviral therapy. Methods A total of 51 samples were collected in Hunan. Viral load and CD4/CD8 counts were determined before antiretroviral drug resistance testing. The sequencing was performed using commercial assays. Genetic mutations associated with drug resistance were identified and interpreted by using the Stanford HIV Drug Resistance Database. Results In PIs resistance mutations one major drug resistance associated mutation V32L, N88D （2.13%, one patient） and two minor mutation A71T （4.26%）, A71V （2.13%） （three patients） were observed. In NRTIs resistance mutations L210M（2.13 % ）, L210F（4.26 % ） （three patients） were observed. In NNRTIs resistance mutations V179D（6.38% ）, V108A（2.13%, four patients） were observed. Conclusions The level of antiretroviral drug resistance among untreated HIV- 1 infection individuals is low. The monitoring of HIV- 1 drug resistance mutations should be paid more attention to it.