高渗盐对心搏骤停鼠脑复苏的作用

Effects of hypertonic saline on cerebral resusctionation after cardiac arrest in rats

目的研究高渗盐对心搏骤停复苏后脑组织的保护作用及其机制,探讨脑复苏治疗的有效方法。方法大鼠窒息导致心搏骤停模型复制成功后,实验两组于复苏即刻分别静脉注射生理盐水、10%高渗盐,比较两组大鼠复苏前及复苏后各时间点平均动脉压值、自主循环恢复(ROSC)时间、动脉血与脑匀浆丙二醛(MDA)、脑干湿质量比、神经功能缺损评分(NDS)及脑海马组织病理改变。结果两组大鼠复苏前平均动脉压值差异无统计学意义(P>0.05),而复苏后各时间点平均动脉压值高渗盐组均高于对照组(P<0.05);高渗盐组较对照组能显著改善自主循环恢复时间(P<0.01),提高24、48hNDS(P<0.05),减轻大脑湿质量(P<0.01)及减轻脑组织病理损伤;但对1h动脉血MDA及24h脑匀浆MDA无作用。结论静脉注射10%高渗盐能减轻大鼠心搏骤停复苏后脑组织损伤,改善脑功能。

Objective To investigate the cerebral protection of hypertonic saline and the mechanism when its solution was applied to the rats of CPR. The modus operandi of cerebral resuscitation was investigated. Methods The SD rats of two groups were intravenously injected with 0.9% NS/10% hypertonic saline respectively after the rat models of asphyxial cardiac arrest were established. Mean arterial pressure （ MAP ）, restoration of spontaneous circulation （ ROSC ） time, arterial blood/brain homogenate MDA, brain dry - weigh/wet - weigh, neurological deficit scores （ NDS ） and the pathological change of the rats hippoeampus were compared. Results Compared with the control group, MAP was increased after cardiac arrest （ P 〈 0.05 ） whereas it was indistinctive before （ P 〉 0.05 ）. ROSC time was decreased greatly （ P 〈 0.01 ） , NDS （ 24 h ,48 h ） were improved （ P 〈 0.05） , brain dry - weigh/wet - weigh were increased （P 〈0.01 ）, cerebral pathological outcome were better in the hypertonic saline group. But blood（ 1 h）/ brain homogenate（24 h） MDA were indistinct（P〉0.05）. Conclusion Intravenous injection of 10% hypertonic saline improves cerebral resuscitation outcome after asphyxial cardiac arrest in rats.