丹参酮ⅡA对小鼠骨髓瘤细胞SP2/0体外增殖与凋亡的影响

Effect of Tanshinone ⅡA on proliferation and apoptosis of SP2/0 cell line in vitro

目的探讨丹参酮ⅡA(TanshinoneⅡA,TanⅡA)对小鼠骨髓瘤细胞SP2/0的生长抑制与诱导凋亡作用。方法选用小鼠骨髓瘤细胞系SP2/0,运用光镜、MTT法、DNA琼脂糖凝胶电泳分析SP2/0细胞增殖及凋亡的改变。结果经丹参酮ⅡA处理后,SP2/0细胞增殖明显被抑制,当4.0μg/mL TanⅡA处理SP2/0细胞72 h,增殖抑制率达(79.4±2.2)%,镜下可见凋亡细胞的形态学改变,细胞DNA片断化(呈现"梯状"断裂现象)。结论TanⅡA能有效地抑制小鼠骨髓瘤细胞系SP2/0增殖,诱导SP2/0细胞凋亡。

Objective To study the effect of Tanshinone lI A on prolixferation and apoptosis of mouse cell line SP2/0 in vitro. Methods The cell proliferation was measured by cell counting and MTT assay. Cell apoptosis was determined by morphology and agarose gel electrophoresis methods, Results After treatment with 0.25 -- 4.0μg/mL Tan ⅡA for 72 h, the proliferation of SP2/0 cells was significantly suppressed, and the morphologic characteristics of apoptotic cells were observed under microscope, DNA ladder was presented in DNA electrophoresis. Conclusion Tan Ⅱ A can effectively inhibit the proliferation and induce the apoptosis of mouse SP2/0 cell in vitro.