摘要
目的建立测定人血浆中司帕沙星的药物浓度的方法。方法采用乙腈萃取,高效液相色谱法测定。色谱柱:Hypersil BDS-C18(4.6 mm×150 mm,5μm);流动相:0.25%磷酸乙腈溶液(450∶100,用三乙胺调pH=2.50);流速:1 mL/min;紫外检测器:波长298 nm,柱温40℃。结果司帕沙星的保留时间为6.1 min,定量线性范围:0.06-2.16μg/mL,方法回收率〉90%(n=5),日内、日间RSD〈10%(n=5)。结论本方法快速,定量准确,可用于血药浓度测定及人体药代动力学研究。
Objective To determine the Sparfloxacin concentration in human plasma. Methods The plasma sample was extracted by acetonitrile and determined by HPLC, the stationary phase was Hypersil BDS-C18 (4.6 mm× 150 mm, 5 μm), the mobile phase consisted of 0.25 % H3PO4 and acetonitrile (450 : 100), pH = 2.50 by spasmolytol. The flow rate was 1.0 mL/min, ultraviolet detector was performed with wavelength detector at 298 nm. Results The retention time of Sparfloxacin was about 6.1 min, and the linear range of quantity was 0.06- 2.16 btg/mL. The recoveries of method were more than 90 % ( n = 5), RSD 〈 10 % ( n = 5). Conclusion This method is rapid and accurate, It can be used for the determination of the content of plasma Sparfloxacin and the study of pharmacokinetics.
出处
《实用药物与临床》
CAS
2007年第4期252-253,共2页
Practical Pharmacy and Clinical Remedies