1Markwardt F.Hirudin as alternative anticoagulant-a historical review[J].Semin Thromb Hemost,2002,28(5):405 -414.
2Harvey R P,Degryse E,Stefani L,et al.Cloning and expression of a cDNA coding for the anticoagulant hirudin from the blood sucking leech:Hirudo medicinalism[J].Proc.Natl.Acad.Sci.USA,1986,83:1084-1088.
3De Taxis du Poet P,Scacheri E,enatti L,et al.Production of the HV1 variant of hirudin by recombinant DNA methodology[J].Blood Coagul Fibrinolysis,1991,2:113.
5Kochanowski R,Kotlowski R,Szweda P.Novel method of expression and purification of hirudin based on pBAD Topo,pTYB12 vectors and gene synthesis[J].Protein Expr Purif,2006,10:24-28.
7Loison G,Courtney M,Lemoine Y,et al.Production and evaluation of recombinant hirudin[J].Semin Thromb Hemost,1989,15:288.
8Kim M D,Rhee S K,Seo J H.Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosmal delta-integration[J].Biotechnol,2001,85:41-48.
9Guo C H,Ohsako T,Ozaki A,Kurosaka A.Production of transplastomic tobacco plants having a gene encoding hirudin[J].Breed Res,2004,6:268.
10Chaudhary S,Parmenter D L,Moloney MM.Transgenic Brassica carinata as a vehicle for the production of recombinant proteins in seeds[J].Plant cell rep,1998,17:195-200.
4Chang JY. The hirudin-binding site of human a-thrombin[J]. J Bio Chem, 1989,264:7141-7146.
5Chang JY. Production, properties, and thrombin inhibitory mechanims of hirudin amino-terminal core fragments[J]. J Bio Chem, 1990,1265: 22159-22166.
6Chang Jui-Yoa.Stability of Hirudin, a Thrombin-specific Inhibitor[J]. Bio Chem,1991,266:10839-10843.
7Timothy J Rydei, Ravichandran KG, Tulinsky A. The structure of a complex of recombinant hirudin and human a-thrombin[J]. Science,1990: 277-280.
8Kou Sreekrishna,Robert G Brankamp.Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris [J]. Gene, 1997,190:55-62.
10TAN S H, WU W T, LIU J J, et al. Efficient expression and secretion of recombinant hirudin Ⅲ in E. coli using the Lasparaginase lI signal sequence [ J ]. Protein Expr Purif, 2002, 25 (3) : 430-436.
