当归芍药散防治老年期痴呆的物质基础与作用机理研究Ⅹ——精简方及其CO_2超临界萃取物对星形胶质细胞缺氧损伤的保护作用

Study Ⅹ on Chemical Substance and Mechanism of Danggui Shaoyao San in the Prevention and Treatment of Senile Dementia——Protective effect of FBD and CO_2 Supercritical Fluid Extract(P_2) on Astrocyte Anoxic Injury

目的：观察当归芍药散精简方（FBD）及其CO2超临界萃取物（P2）对体外培养的大鼠乳鼠皮质星形胶质细胞缺氧损伤的保护作用。方法：SD大鼠po FBD 125mg／kg或P2 16．3mg／kg后，采集含药脑脊液（CSF-FBD／P2）；MTT法测定FBD、P2人工脑脊液（ACSF-FBD／P2）及其含药脑脊液（CSF-FBD／P2）对Na2S2O4诱导星形胶质细胞缺氧损伤的保护作用。结果：1mmol／LNa2S2O4作用1h复氧4h可显著降低星形胶质细胞活力（P〈0．01）；（0．1—1）mg／LACSF．FBD和5％-15％CSF-FBD可显著促进缺氧损伤星形胶质细胞活力（P〈0．01）；（0．01—1）mg／LACSF．P2和15％CSF-P2可极显著或显著促进缺氧损伤星形胶质细胞活力（P〈0．01—0．05）。结论：FBD及其CO2超临界萃取物对星形胶质细胞缺氧损伤具有保护作用。

Objective：To observe the protective effect of FBD, the simplified formula from Danggui Shaoyao San （DSS）, and CO2 supercritical fluid extract（ P2 ） on the hypoxic astrocyte anoxic injury. Methods： Sampling cerebrospinal fluid containing FBD/P2 （CSF-FBD/P2） after administration with FBD 125 mg/kg or P2 16,3 mg/kg; MTI＇ assay was used to measure the viability in the damaged astrocyte induced by Na2 S2 04 Results ： 1 h hypoxia and 4 h reoxygen significantly decreased the viability in astmcyte（P 〈 0.01）;（0.1 - 1 ）mg/L ACSF-FBD and 5% - 15% CSF-FBD can substantially increase the viability of astrocyte induced by Na2S204 （P 〈 0.01） ; （0.01 - 1） mg/L ACSF-P2 and 15% CSF-P2 can substantially increase the viability of astrocyte（ P 〈 0.01 - 0.05）. Conclusion： FBD and P2 could prevent astrocyte from Na2S204-induced hypoxic damage,which was beneficial to the treatment of cerebral ischemia.