摘要
目的:经对不同纯化工艺制备的荭草提取物进行药效学比较研究,确定合理可行的精制纯化工艺。方法:采用结扎大鼠冠状动脉左前降支建立急性心肌缺血模型,股静脉给药比较D101大孔吸附树脂吸附分离与正丁醇萃取两种工艺的不同提取部位对急性心肌缺血大鼠心电图、心肌梗死范围、乳酸脱氢酶(LDH)、肌酸激酶(CK)的影响。结果:与大孔吸附树脂比较,荭草的正丁醇提取物能更好地抑制急性心肌缺血大鼠心电图J点升高,减小心肌梗死范围,降低CK、LDH水平。结论:荭草采用正丁醇提取能够将有效成分充分富集。
Objective：To compare Pharmacodynamics properties between the extracts of Hongcao, and determine an optimum technique to refine and purify. Methods anterior branch of the left coronary artery. The change ：The acute myocardial infarction of rat was induced by ligating of ECG, and the level of CK, LDH were determined, the infarction area of myocardium was also observed. Results：N-butanol extracts of Hongcao could obviously decrease the elevated J of ECG,decrease the infarction area of myocardium, reduce the level of CK, LDH. Conclusion： Adopt n-butanol extraction can concentrate effective composition of Hongcao.
出处
《中国实验方剂学杂志》
CAS
2007年第8期21-24,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省中药现代化科技产业研究开发专项基金项目(黔科合中药专字[2004]03号)
关键词
荭草
急性心肌缺血
大孔吸附树脂
正丁醇
Hongcao
myocardial ischemia
macroporous absorbing resin
n-butanol
