三七、绞股蓝抗兔血管重构的实验研究

Effect of Panax notoginseng(San Qi) and Gynostemma pentaphyllum(Jiao Gu Lan) on Vascular Remodeling in Rabbits

目的：研究三七、绞股蓝单方及配伍方对实验性兔动脉粥样硬化（AS）模型血管重构的影响。方法：64只3—4月龄新西兰雄兔随机分为正常组（Contr01）、模型组（Model）、三七组（sQ）、绞股蓝组（JGL）、复方组（FF）、卡托普利组（Captopril）。除正常对照组外，均予耳缘静脉注射牛血清白蛋白250mg／ks后继喂高脂饲料，同时各药物组给予相应的药物，连续12周。于12周末行胸主动脉HE、EF（弹力纤维）染色，观测VSMC（血管平滑肌细胞）核个数、EF的变化；免疫组织化学标记胸主动脉PCNA（增殖细胞核心抗原）、TGF—β1（转化生长因子-β1）表达。结果：（1）与模型组比较，各药物组主动脉病变不同程度减轻，尤以复方组为优；（2）复方组与模型组比较VSMC核个数显著下降；（3）模型组与正常组比较，主动脉PCNA数密度百分数、TGF—β1面积百分比显著升高（P〈0．01），各药物组有不同程度的降低。结论：三七、绞股蓝及复方通过降低TGF—B1表达，减轻该因子致细胞增殖的作用，减轻血管壁肥厚性重构。

Objective： To investigate the effects and mechanisms of Panax notoginseng （SQ） and Gynostemma pentaphyllum（JGL） on vascular remodeling. Methods： A total of sixity-four male New Zealand rabbits was randomly divided into six groups：Normal, Model, SQ, JGL, FF（SQ ＋ JGL） and Captopril. The rabbits in control group were fed with regular diet, others were injected with bovine serum albumin 250 mg/kg and fed with high cholesterol diet, meanwhile, durgs were administrated orally. Rabbits were sacrificed at the end of 12 week,aortas serial cross-sections were stained with hematoxylin-eosin and elastic fibers. Immunohistochemistry were performed to label PCNA and TGF-β1. Results：（1） The pathological changes of all drug treated groups were alleviated. （2）Compound prescription decreased the quantity of aorta VSMC nucleus compared with model group（ P 〈 0.05）. （3）The aorta PCNA immunostaining number and TGF-β1 immunostaining area of model group were significantly higher than normal group （ P 〈 0.01 ）, all drug treated groups decreased. Conclusion：Reduced expression of TGF-β1 ,and cellular proliferation may be related with inhibition of VR.