姜黄素对裸鼠乳腺移植瘤p21及CD44V_6表达的影响

Effect of curcumin on expression of p21 and CD44V_6 in human breast cancer xenografts

目的:研究姜黄素对裸鼠乳腺移植瘤p21及CD44V6表达的影响。方法:选用人乳腺癌细胞株MCF-7对裸鼠进行异种移植,成瘤后随机分为2组:(1)阴性对照组;(2)姜黄素组。观测移植瘤的出瘤时间、成瘤率,测量瘤体大小并计算瘤表面积。同时应用RT-PCR,检测2组肿瘤组织中cyclin D1、p21及CD44V6的表达。结果:姜黄素组瘤表面积明显低于阴性对照组;姜黄素组p21表达量高于阴性对照组,CD44V6表达量明显降低,2组的cyclin D1表达差异无显著。结论:姜黄素抑制裸鼠MCF-7乳腺移植瘤CD44V6的表达,增加p21的表达。

AIM ： To study the effect of curcumin on the expression of p21 and CD44V6 in breast carcinoma in nude mice. METHODS ： Nude mice were xenografted with human breast cancer cell line MCF - 7 and randomly divided into 2 groups （ n =4 in each group） ： control group and curcumin group. In latent period, the percentage of tumor development was observed. Tumors were measured and the surface areas were calculated. RT - PCR was performed to detect the expression level of cyclin D1, p21 and CD44V6 mRNA. RESULTS： The tumor surface areas in the curcumin group were significantly lower than those in control group. In curcumin treatment group, the expression of p21 was up - regulated while cyclin D1 was nearly not changed. The expression of CD44V6 was significantly down - regulated in curcumin group. CONCLUSION ： Curcumin inhibits the expression of CD44V6 and up - regulates the expression of p21 in nude mice beating hu- man breast cancer cell line MCF -7.