人参二醇组皂苷对脂多糖攻击大鼠脑皮质NF-κB的影响

Effects of PDS on rat brain cortical nuclear factor kappa B in LPS shock

目的:观察人参二醇组皂苷对脂多糖(LPS)攻击大鼠脑皮质NF-κB的影响。方法:大鼠随机分为实验对照(control)组、脂多糖(LPS)组、脂多糖加地塞米松(LPS+Dex)组和脂多糖加人参二醇组皂苷(LPS+PDS)组。大鼠静脉注射LPS(4 mg.kg-1)1 h、4 h后检测脑皮质NF-κB的变化。结果:EMSA实验显示人参二醇组皂苷可抑制LPS攻击后1 h、4 h NF-κB的DNA结合活性;抑制细胞核p65和p50蛋白的表达。结论:LPS休克早期脑组织细胞NF-κB激活入核,人参二醇组皂苷通过抑制其活性而减轻脑组织损伤。

AIM ： To explore the molecular mechanism of brain tissue injury induced by lipopolysaccharide （LPS）, the effects of panaxadiol （PDS） on the expression of nuclear factor kappa B （NF -κB ） in cerebral cortex of rat with LPS shock were studied. METHODS： Rats were randomly divided into LPS group, LPS ＋ dexamethasone group, LPS ＋ PDS group and control group. The DNA binding activity and protein expression of NF -κB were observed. These indices were assayed at 1 h and 4 h after intravenous injection of LPS （4 mg· kg^-1 ）. RESULTS ： EMSA showed that PDS inhibited NF -κB DNA - binding activity in nuclear extracts at both 1 h and 4 h after LPS injection, compared with the LPS group （ P 〈0.01 ）. Western blotting showed that PDS down - regulated the expression of p65 and p50 protein in the nuclear extracts compared with the LPS group. However, the expression of p65 and p50 protein from cytosolic extracts did not show any significant change. CONCLUSION ： PDS may alleviate brain injury by inhibiting NF -κB activation.