慢性重型乙型肝炎患者外周血单核细胞Toll样受体4的变化及其与IL-6的关系

Correlation between Toll-like receptor 4 on peripheral blood monocytes and serum IL-6 in patients with chronic severe hepatitis B

目的:探讨慢性重型乙型肝炎患者外周血单核细胞Toll样受体4(TLR4)的变化情况及其意义。方法:用流式细胞仪检测30例正常对照、31例慢性乙型肝炎患者和30例慢性重型乙型肝炎患者外周血单核细胞表面TLR4的表达,ELISA法检测上述患者血清白细胞介素6(IL-6)的水平。结果:正常对照组、慢性乙型肝炎组和慢性重型乙型肝炎组外周血单核细胞TLR4的平均免疫荧光强度分别为2.3±1.1、3.7±2.3和6.9±4.1,慢性重型乙型肝炎组外周血单核细胞TLR4表达显著高于正常对照组和慢性乙型肝炎组(P<0.05),慢性乙型肝炎组与正常对照组比较无统计学差异;3组外周血清IL-6水平分别为(11.5±7.2)ng/L、(40.8±31.2)ng/L和(77.6±33.3)ng/L,各组间比较均具有显著差异(P<0.05);慢性重型乙型肝炎组外周血单核细胞TLR4表达水平与血清IL-6表达水平呈显著正相关,相关系数γ=0.618,P<0.05。结论:TLR4可能与慢性乙型肝炎重型化有关。

AIM ： To study the change of Toll-like receptor 4 （TLR4） on peripheral blood monocytes （PBMCs） and its role in the pathogenesis of chronic severe hepatitis B, METHODS ： The expression of TLR4 on CD14 ^＋ PBMCs was determined by flow cytometry in 30 healthy control, 31 patients with chronic hepatitis B and 30 patients with chronic severe hepatitis B, The level of serum interleukin - 6 （ IL - 6） was detected by ELISA, RESULTS ： The expressions of TLR4 on PBMCs and serum IL -6 in the groups of healthy control, patients with chronic hepatitis B and patients with chronic severe hepatitis B were 2. 3 ± 1. 1, 3. 7 ± 2. 3, 6. 9 ±4.1 mean fluorescence intensity （MFI） and （11.5 ±7. 2） ng/L, （40. 8 ± 31.2） ng/L, （77. 6 ± 33. 3 ） ng/L, The TLR4 value in the group of patients with chronic severe hepatitis B was significant higher than that in the group of healthy control and the group of patients with chronic hepatitis B （ P 〈 0. 05 ）, However, there was no significant difference between the group of patients with chronic hepatitis B and the group of healthy control （ P 〉 0, 05 ）. Serum IL - 6 increased gradually and significantly between the group of healthy control and the groups of patients with chronic hepatitis B and patients with chronic severe hepatitis B. There was a significant positive correlation between the expression of TLR4 and the content of serum IL - 6 in the group of chronic severe hepatitis B. CONCLUSION： TLR4 may play a role in the pathogenesis of chronic severe hepatitis B.