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大鼠视网膜干细胞体外培养、增殖及分化

Incubation,proliferation and differentiation of retinal stem cell in the rat eyes
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摘要 目的:大鼠视网膜干细胞的培养及鉴定。方法:用神经干细胞无血清培养方法从出生10 d的大鼠睫状体部分离出具有单细胞克隆能力的细胞球,用MTT法检测单克隆细胞增殖能力,应用免疫组织化学的方法检测干细胞分化特性。结果:所获得的细胞球具有连续克隆的能力,nestin抗原阳性,分化后的细胞表达星形胶质细胞、神经元、感光细胞和双极细胞特异性抗原。结论:成功培养出大鼠视网膜干细胞。
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2007年第8期1639-1641,共3页 Chinese Journal of Pathophysiology
关键词 视网膜干细胞 细胞培养 细胞分化 大鼠 Retinal stem cells Cell culture Cell differentiation Rats
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参考文献7

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