卵巢浆液性癌中K-ras基因突变和p21、p53蛋白的表达

K-ras mutations and expression of p21, p53 proteins in ovarian serous carcinoma

目的研究卵巢浆液性癌及交界性病变中的K-ras基因第1外显子第12号密码子的点突变及p21和p53蛋白的表达,探讨其与卵巢浆液性癌癌变的关系。方法应用显微切割、PCR-RFLP和免疫组织化学技术,对30例卵巢浆液性癌及其交界性病变区的K-ras基因突变及p21和p53蛋白表达进行研究。结果卵巢浆液性癌K-ras基因突变率为17.8%,交界性病变为12.5%,良性病变未检测出突变。浆液性癌p53蛋白表达率为66.7%,明显高于交界性病变的37.5%。p21蛋白在浆液性癌的表达率为33.3%,明显低于交界性病变的87.5%。结论K-ras基因突变主要发生于低分级浆液性癌中,是其发生的早期分子事件。p53和p21蛋白在浆癌中表达呈明显的负相关,提示突变型p53基因失去诱导p21的基因表达及其负性调控作用可能是导致细胞持续恶性增殖而癌变的原因。

Objective To study K-ras mutations and expression of p21, p35 proteins in ovarian carcinoma, and its relationships. Methods K-ras mutations and expressions of p21 and p53 proteins were detect- ed in 30 cases of ovarian serous carcinomas with microdissection technique and PCR-RFLP and immunohisochemical techniq-ue. Results The expression of p53 in ovarian carcinoma was 66.7%, that significantly higher than borderline malignancy. While p21 protein in ovarian carcinoma was 33.3%, that significantly low- er than borderline malignancy. Conclusions K-ras mutations in serous ovarian carciuoma are very few and correlated with the low-grade carcinoma, p53 gene is mostly correlated with the high-grade ovarian carcinoma through losing inducement with p21 gene.