摘要
本实验在中试水平上研究生物酶解技术提取鲜沙姜精油的设备与工艺。所设计的设备包括酶解、水蒸汽蒸馏提取、萃取及浓缩四种功能。研究内容涉及到调整设备参数、设定四个影响提取率的关键因素范围;采用正交试验优化提取工艺并以GC-MS法检测并评价产品。实验结果如下:当淀粉液化酶用量为3.0‰,固液比为20%,提取时间为4h,萃取剂石油醚与馏出液比例为1:8时,完全消除了冲浆对提取过程的影响;使油水混合物的馏出时间从3.5h降到2.0h;精制后精油提取率上升约9%;经GC-MS检测,精油中主要成分沙姜脑的含量提高约15%。
The equipment and technics on the pilot experiment were studied in this paper for extracting the essential oil in Kaempferia galanga L. by enzymatic hydrolysis-hydro distillation. The designed equipment possesses four functions which areenzymatic hydrolysis, hydro distillation （HD）, extraction and concentration. The contents we studied involved in adjusting parameters for the equipment, selecting the range of the four factors which affected the yield, optimizing the technics by orthogonal design, and testing and appreciating the product by GC-MS. The results were as follows： when the dosage of the liquefying enzyme 0.3%, solid-to-liquid ratio 20%, extracting time 4 h, and the ratio of petroleum ether to distillate 1：8, the viscosity of the HD system decreased greatly, and the Kaempferia galanga L. slurry running out of the extracting pot could be avoided. The distilling time decreased from 3.5 h to 2.0 h, and the yield of refmed essential oil increased about 9%. Determination results of GC-MS showed that and the content of the main component in the oil increased about 15%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第8期184-188,共5页
Food Science
基金
国家级星火计划项目(2005EA780029)
广东省科技计划项目(2006B20901006)
广州市科技计划项目(2005Z3-E0561)
关键词
沙姜精油
酶解
中试
正交试验
水蒸汽蒸馏
essential oil in Kaempferia Galanga L.
enzymatic hydrolysis
pilot experiment
orthogonal design
hydro distillation
