碱溶性菜籽多糖的提取条件优化及体外抗氧化活性研究

Optimization of Technology for Extracting Alkali-soluble Polysaccharides from Rapeseed Meal and Study on Anti-oxidative Effect in vitro

本实验采用氢氧化钠提取菜籽饼粕中的多糖,选定提取温度、浸提液浓度、提取时间三因素三水平进行中心组合试验,建立了菜籽多糖提取的二次回归方程。通过SAS软件做响应面分析以及岭嵴分析得到菜籽多糖氢氧化钠提取的最佳条件。试验结果表明,当提取工艺条件为:提取温度93.7℃,浸提液浓度1.124mol/L,提取时间2.3h,其多糖含量为11.59%。并对碱溶性菜籽多糖进行了体外抗氧化活性研究,表明菜籽多糖有一定抗氧化生物活性。

In this paper, sodium hydroxide was used as extracting agent from rapeseed meal. Three levels of the factors including extraction temperature, concentration of NaOH and extraction time were selected for the central composite design experiment. The quadric regression model for the yield of polysaccharides was established. Then the optimum combination was obtained by SAS analysis and response surface analysis. Experimental results indicated that when the extraction parameters are composed of 93.7℃, 1.125 mol/L and 2.3 h, the yield of polysaccharides is maximum to 11.59%. At last the study of polysaccharide from rapeseed meal anti-oxidation was conducted, and the medical valve of rapeseed meal was proved.