摘要
为得到兔血清中纯度较高的特异性抗体,通过将纯化的水牛乳β-乳球蛋白耦联到免疫亲和柱上,用3mol/L氯化镁将特异性的IgG洗脱,得到了SDS-PAGE纯的IgG,其蛋白含量为5.8mg/ml,经ELISA检测滴度为1:109。本实验所建立的方法可用于纯化兔血清中特异性的IgG。
In order to collect high purity rabbit antibody against buffola＇s β-1actoglobulin, β-1actoglobulin was coupled into the medium of Sepharose 4]3, and then the medium was eluted with 3 mol/L MgCh. The results showed that electrophoretically pure of IgG is identified by SDS-PAGE, the protein content is 5.8 mg/ml, and the titer is 1：109 using ELISA. So the method established can be used to purify the specific IgG in rabbit serum.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第8期276-279,共4页
Food Science
基金
国家自然基金项目(30560096)
长江学者和创新团队发展计划项目(IRT0540)
